Dr Trong Khoa Pham

MSc, PhD, FHEA

School of Biosciences

Biological Mass Spectrometry Facility Manager

Expertise: Proteomics

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Khoa_portrait
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khoa.pham@sheffield.ac.uk
+44 114 222 4676
B11f, Dainton Building

Full contact details

Dr Trong Khoa Pham
School of Biosciences
B11f
Dainton Building
13 Brook Hill
Sheffield
S3 7HF
Research interests

With over fifteen years of experience in biological mass spectrometry analyses, I am passionate about using these tools to enhance our understanding and improving of biological processes, and discover new biomarkers.

I specialise in proteomics analysis, including method development, identification and characterization of both small and large molecules (metabolites, peptides, and proteins), as well as post-translational modification (PTM) analysis (e.g. phosphorylation, methylation, acetylation, and glycosylation), relative and absolute quantitation (LFQ and multiplexing), and protein interaction in biological samples (specimens).

Throughout my career, I have collaborated with researchers and academics on numerous projects, contributing to many successful initiatives. I am always looking to expand my collaborative networks, both nationally and internationally, and welcome opportunities to work with partners to advance our understanding of the complex biological systems that shape our world.

Publications

Journal articles

  • English DM, Lee SN, Sabat KA, Baker IM, Pham TK, Collins MO & Cowley SM (2025) . Nucleic Acids Research, 53(4).
  • Bui PT, Pham KT & Vo TDL (2023) . Processes, 11(8).
  • Pham TK, Buczek WA, Mead RJ, Shaw PJ & Collins MO (2021) . Frontiers in Molecular Neuroscience, 14.
  • Hounslow E, Evans CA, Pandhal J, Sydney T, Couto N, Pham TK, Gilmour DJ & Wright PC (2021) . Biotechnology for Biofuels, 14(1).
  • Benninghoff JC, Kuschmierz L, Zhou X, Albersmeier A, Pham TK, Busche T, Wright PC, Kalinowski J, Makarova KS, Bräsen C , Flemming H-C et al (2021) . Applied and Environmental Microbiology, 87(11).
  • Vo TDL, Pham KT & Doan KT (2021) . Waste and Biomass Valorization, 12(3), 1565-1579.
  • Qiu W, Evans CA, Landels A, Pham TK & Wright PC (2020) . Analytica Chimica Acta, 1129, 158-180.
  • Vo TDL & Pham KT (2020) . International Journal of Food Engineering, 16(4).
  • Vo TDL, Pham KT, Le VMV, Lam HH, Huynh ON & Vo BC (2020) . Process Biochemistry, 91, 374-386.
  • Raut MP, Pham TK, Gomez LD, Dimitriou I & Wright PC (2019) . Biomass and Bioenergy, 130, 105382-105382.
  • Lindblad P, Fuente D, Borbe F, Cicchi B, Conejero JA, Couto N, ÄŒeleÅ¡nik H, Diano MM, Dolinar M, Esposito S , Evans C et al (2019) . Algal Research, 41.
  • Hoffmann L, Anders K, Bischof LF, Ye X, Reimann J, Khadouma S, Pham TK, van der Does C, Wright PC, Essen L-O & Albers S-V (2019) . Journal of Biological Chemistry, 294(18), 7460-7471.
  • Vo TDL, Pham KT, Le LT & Nguyen TTH (2018) . Journal of Food Processing and Preservation, 42(12), e13837-e13837.
  • Frey AM, Ansbro K, Kamble NS, Pham TK & Stafford GP (2018) . FEMS Microbiology Letters, 365(17).
  • Qiu W, Pham TK, Zou X, Ow SY & Wright PC (2017) . Journal of Proteome Research, 16(7), 2370-2383.
  • Stark H, Wolf J, Albersmeier A, Pham TK, Hofmann JD, Siebers B, Kalinowski J, Wright PC, Neumann-Schaal M & Schomburg D (2017) . The FEBS Journal, 284(13), 2078-2095.
  • Wolf J, Stark H, Fafenrot K, Albersmeier A, Pham T, Muller KB, Meyer B, Hoffmann L, Shen L, Albaum SP , Kouril T et al (2016) . Molecular Microbiology, 102(5), 882-908.
  • Fowler GJ, Pereira-Medrano AG, Jaffe S, Pasternak G, Pham TK, Ledezma P, Hall ST, Ieropoulos IA & Wright PC (2016) . Proteomics, 16(21), 2764-2775.
  • Esser D, Hoffmann L, Pham T, Bräsen B, Qiu W, Wright PC, Albers S-V & Siebers B (2016) . FEMS Microbiology Reviews.
  • Raut MP, Couto N, Pham TK, Evans C, Noirel J & Wright PC (2016) . Biotechnology for Biofuels, 9(1).
  • Wase N, Pham TK, Ow SY & Wright PC (2014) . Journal of Proteomics, 109, 332-355.
  • Kort JC, Esser D, Pham TK, Noirel J, Wright PC & Siebers B (2013) . Proteomics, 13(18-19), 2831-2850.
  • Reimann J, Esser D, Orell A, Amman F, Pham TK, Noirel J, LindÃ¥s A-C, Bernander R, Wright PC, Siebers B & Albers S-V (2013) . Mol Cell Proteomics, 12(12), 3908-3923.
  • Pereira-Medrano AG, Knighton M, Fowler GJS, Ler ZY, Pham TK, Ow SY, Free A, Ward B & Wright PC (2013) . J Proteomics, 78, 197-210.
  • Esser D, Pham TK, Reimann J, Albers SV, Siebers B & Wright PC (2012) . Journal of Proteome Research, 11(10), 4823-4833.
  • Zou X, Pham TK, Wright PC & Noirel J (2012) . Genomics, 100(4), 240-244.
  • Evans C, Noirel J, Ow SY, Salim M, Pereira-Medrano AG, Couto N, Pandhal J, Smith D, Pham TK, Karunakaran E , Zou X et al (2012) . Anal Bioanal Chem, 404(4), 1011-1027.
  • Koerdt A, Orell A, Pham TK, Mukherjee J, Wlodkowski A, Karunakaran E, Biggs CA, Wright PC & Albers SV (2011) . J PROTEOME RES, 10(9), 4105-4119.
  • Bewley MA, Pham TK, Marriott HM, Noirel J, Chu H-P, Ow SY, Ryazanov AG, Read RC, Whyte MKB, Chain B , Wright PC et al (2011) . Mol Cell Proteomics, 10(6), M111.008193.
  • Noire J, Evans C, Salim M, Mukherjee J, Ow SY, Pandhal J, Pham TK, Biggs CA & Wright PC (2011) . Current Proteomics, 8(1), 17-30.
  • Pham TK, Roy S, Noirel J, Douglas I, Wright PC & Stafford GP (2010) . Proteomics, 10(17), 3130-3141.
  • Pham TK, Sierocinski P, van der Oost J & Wright PC (2010) . J Proteome Res, 9(2), 1165-1172.
  • Zaparty M, Esser D, Gertig S, Haferkamp P, Kouril T, Manica A, Pham TK, Reimann J, Schreiber K, Sierocinski P , Teichmann D et al (2010) . EXTREMOPHILES, 14(1), 119-142.
  • Albers SV, Birkeland NK, Driessen AJM, Gertig S, Haferkamp P, Klenk HP, Kouril T, Manica A, Pham TK, Ruoff P , Schleper C et al (2009) . BIOCHEMICAL SOCIETY TRANSACTIONS, 37, 58-64.
  • Pham TK & Wright PC (2008) . J Proteome Res, 7(11), 4766-4774.
  • Pham TK & Wright PC (2008) . J Proteome Res, 7(2), 515-525.
  • Pham TK & Wright PC (2007) . Expert Rev Proteomics, 4(6), 793-813.
  • Chong PK, Gan CS, Pham TK & Wright PC (2006) . J Proteome Res, 5(5), 1232-1240.
  • Pham T, Chong PC, Gan CS & Wright PC (2006) Proteomic analysis of Saccharomyces cerevisiae under high gravity fermentation conditions. J Proteome Res, 5(12), 3411-3419.
  • Bischof LF, Haurat MF, Hoffmann L, Albersmeier A, Wolf J, Neu A, Pham TK, Albaum SP, Jakobi T, Schouten S , Neumann-Schaal M et al () . Frontiers in Microbiology, 9.
  • Sadroddiny E, Ai J, Carroll K, Pham TK, Wright PC, Pathak A & Helm B () Protein Profiling of the Secretome of FcεRI Activated RBL-2H3.1 Cells. Iran J Immunol, 9(1), 1-31.

Conference proceedings

  • Pham TK & Wright PC (2008) Quantitative proteomic analysis of membrane proteins based on gel and shotgun techniques. Aiche Annual Meeting Conference Proceedings

Posters

  • Pham T, Esser D, Reimann J, Qiu W, Ow SW, Albers SV, Siebers B & Wright PC (2012) Improved understanding of protein phosphorylation in Sulfolobus species. 60th ASMS Conference on Mass Spectrometry and Allied Topics Vancouver, BC, Canada.
  • Pham T, Sierocinski P, van der Oost J & Wright PC (2010) Quantitative proteomic analysis of the archaeon Sulfolobus solfataricus P2: Hot life at lower temperatures. 4th EuPA meeting, Estoril, Portugal.
  • Pham T, Sierocinski P, van der Oost J & Wright PC (2010) Regulation of central carbon metabolism in the archaeon, Sulfolobus solfataricus P2 under temperature variations: A quantitative proteomic aspect. BESG Young Researchers Meeting, Institute of Chemical Engineers (IChemE), University of Cambridge, U.
  • Pham T, Sierocinski P, van der Oost J & Wright PC (2009) Proteomic analysis of Sulfolobus solfataricus P2. 2nd SYSMO Evaluation Conference, Vienna, Austria.
  • Pham T, Sierocinski P, van der Oost J & Wright PC (2009) Sulfolobus solfataricus: The life under high temperature. BESG Young Researchers Meeting, IChemE, ±¬ÁÏTV, UK.

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